The three England based players missed the game against South Africa due to issues related to the COVID-19 pandemic as the rainbow country has been declared a COVID-19 risk zone by the World Health Organization (WHO)
The trio trained with the rest of the squad on Friday, at Accra sports stadium as the team began preparation for the final Group C Africa Cup of Nations qualifier against São Tomé and Principe.
Ghana aim for a win on Sunday to keep hold of the top spot and also to finish the qualifiers on a sound footing.
The Black Stars qualified for a record ninth straight Africa Cup of Nations tournament after drawing 1-1 with South Africa on Thursday.
Goalkeepers: Razak Abalora, Danlad Ibrahim, Eric Ofori Antwi
Defenders: Benson Annan, Baba Abdul Rahman, Moro Ibrahim, Christopher Opoku, Ismail Ganiyu, Yussif Mubarik, Joseph Agyei
Midfielders: Thomas Partey, Justice Blay, Gladson Awako, Mubarak Wakaso, Mohammed Kudus, Adubofuor Kwame Opoku, Emmanuel Lomotey, Afriyie Acquah
Forwards: Andre Ayew, Jordan Ayew, Kwame Opoku, Emmanuel Boateng, Caleb Ekuban, Joseph Esso, Osman Bukari, John Antwi, Kwasi Okyere Wreidt, Emmanuel Gyasi.